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Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

Journal: Acta Otorhinolaryngologica Italica

Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

doi: 10.14639/0392-100X-A1222

Figure Lengend Snippet: Effect of IL-29 on eosinophil inflammatory cytokine secretion. (A) ELISA analysis of IL-5 secretion in eosinophil supernatants; (B) ELISA analysis of IL-13 secretion in eosinophil supernatants. Eosinophils were stimulated with different concentrations of IL-29 (10, 25, 50, 100 ng/mL) for 24 hours. Data were obtained from 3 independent experiments and are presented as mean ± SD. * indicates p < 0.05.

Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

Techniques: Enzyme-linked Immunosorbent Assay

The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

Journal: Acta Otorhinolaryngologica Italica

Article Title: Dose-dependent IL-29 activation of TLR4 signalling drives eosinophil infiltration in chronic rhinosinusitis with nasal polyps

doi: 10.14639/0392-100X-A1222

Figure Lengend Snippet: The interventional effect of TAK-242 Blocking TLR4 on IL-29 action. (A) Transwell assay showing changes in eosinophil migration after TLR4 blockade with TAK-242; (B) ELISA of IL-5 secretion after TAK-242 intervention; (C) ELISA of IL-13 secretion after TAK-242 intervention; (D) Western blot analysis of TLR4, p-NF-κB, and p-MAPK protein expression after TAK-242 treatment. Data are presented as mean ± SD. * indicates p < 0.05.

Article Snippet: A total of 1×10 5 eosinophils were seeded in the upper chamber, while the lower chamber was filled with RPMI-1640 medium containing 10 ng/mL IL-5 (R&D Systems, 205-IL-010) as a chemoattractant.

Techniques: Blocking Assay, Transwell Assay, Migration, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing